Producción Científica Profesorado

Determination of antibiotics in feedstuff samples by microemulsion electrokinetic chromatography using fullerene as additive



Rodríguez Ávila, José Antonio

2018

Nieto, N.K., Rodriguez, J.A., Ibarra, I.S., Cruz-Borbolla, J., Vasquez-Perez, J.M., Electrophoresis, (2018), 39, 2228-2235. DOI: 10.1002/elps.201800094


Abstract


A microemulsion electrokinetic chromatography method was developed for the simultaneous detection and quantification of ciprofloxacin, norfloxacin, sulfamethoxazole, tetracycline, and oxytetracycline in feedstuff samples. The BGE composition was optimized by applying a Taguchi parameter design and consisted of phosphate 30 mmol/L, Tween?80 0.01 mmol/L, and fullerene 3 ?mol/L, and adjusted to pH 8.0; the addition of surfactant and fullerene modifies the mobility of the analytes improving their resolution. Theoretical studies showed ??? and van der Waals interactions between antibiotic molecules and fullerene used as a pseudostationary phase. Under optimal conditions, limits of detection ranged from 0.7 to 1.5 ?g/g; the analytes were separated in less than 6 min. The methodology proposed is useful for controlling and monitoring antibiotic residues in feedstuff samples.



Producto de Investigación




Artículos relacionados

Presence and antimicrobial resistance of Escherichia coli isolated from foodstuffs in Hidalgo State ...

Potentiometric Behavior of Diverse Polypyrrole-sulphate Films Electro Synthesized on Graphite - Epox...

Determination of primary amino acids in wines by high performance liquid magneto-chromatography

Silver and Silver Chloride Electrodeposits, an Alternative in the Construction of Ag/AgCl Solid Elec...

Evaluación del contenido de metales y su efecto en la estabilidad de antocianinas

Validation of a tubular bismuth film amperometric detector: Determination of diclofenac sodium by mu...

Chemical studies of anthocyanins: A review

Construction and Evaluation of a Gold Tubular Electrode for Flow Analysis: Application to Speciation...

Design and Construction of Solid State Ag/AgCl Reference Electrodes Through Electrochemical Depositi...

Potentiometric Behavior of Diverse Polypyrrolesulphate Films Electro Synthesized on Graphite Epoxy ...