Producción Científica Profesorado

Size exclusion chromatography (SEC-HPLC) as an alternative to study thrombin inhibition



Alvarez Romero, Giaan Arturo

2018

Size exclusion chromatography (SEC-HPLC) as an alternative to study thrombin inhibition, Pérez-Escalante, E., González-Olivares, L.G., Cruz-Guerrero, A.E., Galán-Vidal, C.A., Páez-Hernández, M.E., Álvarez-Romero, G.A., Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1074-1075 (2018) 34-38, DOI: 10.1016/j.jchromb.2017.12.037


Abstract


In vitro analysis of anticoagulant compounds with a potential use as antithrombotic drugs, has been traditionally performed using techniques like spectrophotometry, turbidimetry, as well as electrochemical and clinical assays. Although, these techniques have some disadvantages such as: the inability to measure the total biological activity of thrombin, interferences and, sometimes, the quantitative determination of the inhibition ratio is not accurate. In the present work, the conversion of fibrinogen to fibrin was monitored by molecular exclusion chromatography (SEC-HPLC) in three different reaction systems. An inhibition percentage of 43.192.02% was obtained using heparin as an anticoagulant, in addition to the determination of the percentage of heparin bonded to thrombin. This methodology has not been previously described and has high potential for the determination of anticoagulant capacity with higher precision, the determination of thrombin's total biological activity and the quantitative determination of the inhibition ratio



Producto de Investigación




Artículos relacionados

Synthesis and crystal structures of cis-palladium(II) and cis-platinum(II) complexes containing dipy...

Sequential Injection Spectrophotometric Determination of Diclofenac in Urine and Pharmaceutical Form...

Construction and Evaluation of a Gold Tubular Electrode for Flow Analysis: Application to Speciation...

Magnetic solids in analytical chemistry: A review

Determination of tetracyclines in milk samples by magnetic solid phase extraction flow injection ana...

Metal content evaluation and its effect on the stability of anthocyanins

Potentiometric behavior of graphite-epoxy electrochemical transducers towards anions, cations and pH...

Electrochemical and AFM characterization of the electropolimerization of pyrrole over a graphiteepox...

Development of a novel nitrate-selective composite sensor based on doped polypyrrole

Estudio de las Interacciones Ácido Húmico-Metales Pesados y Determinación de sus Constantes de Estab...