Producción Científica Profesorado

Determination of glyphosate and aminomethylphosphonic acid in soils by HPLC with pre-column derivatization using 1, 2-naphthoquinone-4-sulfonate.



Mendoza Huizar, Luis Humberto

2014

Determination of glyphosate and aminomethylphosphonic acid in soils by HPLC with pre-column derivatization using 1, 2-naphthoquinone-4-sulfonate. Gabriela Islas, José A. Rodriguez, Luis H. Mendoza-Huizar, Fidel Pérez-Moreno, E. Gabriela E. Carrillo. Journal of Liquid Chromatography & Related Technologies, 37:1298?1309, 2014. ISSN 1082-6076 (Print), 1520-572X (Online).


Abstract


A simple selective high-performance liquid chromatography (HPLC) method has been developed for the determination of glyphosate and its main metabolite aminomethylphosphonic acid residues in soil samples based on a pre-column derivatization with 1,2-naphthoquinone-4-sulfonate (NQS). The reaction is performed at pH 9.4 and 60C for 5 min. The separation of glyphosate was carried out in a C18 column using a 1% acetic acid?methanol (40:60, v/v) mobile phase and UV detection. An experimental design approach based on the central composite design is used to investigate the dependence of the derivatization variables of pesticide. The concentration of NQS and reaction temperature were found to be statistically significant. The limits of detection obtained were 0.064 mg kg?1 for glyphosate and 0.098 mg kg?1 for its metabolite. The proposed method was applied successfully to the analysis of real soil samples from corn crops. The proposed method is easier and faster compared to other ones.



Producto de Investigación




Artículos relacionados

Efecto de la composición del soporte sobre las estructuras superficiales de óxidos de níquel y molib...

THEORETICAL STUDY ON THE MODIFICATION OF ACTIVE SITES IN GOLD SURFACES MODELED AS FINITE CLUSTERS: I...

A theoretical quantum study on the distribution of electrophilic and nucleophilic active sites on th...

Evaluación de Esteroides Aromáticos como Indicadores de Madurez Térmica en Extractos de Roca

A Semiempirical PM6 Study of Some Aminopyrimidine Derivatives and their Interaction with an Iron Sur...

Synthesis of Zn compounds derived from 1H-benzimidazol-2-ylmethanamine

Hydrogen bond studies in substituted N-(2-hydroxyphenyl)--2-[(4-methylbenzenesulfonyl)amino]acetamid...

Characterization of Main Anthocyanins Extracted from Pericarp Blue Corn by MALDI-ToF MS

Morphological and magnetic properties of cobalt nanoclusters electrodeposited onto HOPG

Nucleation and growth of cobalt onto different substrates: Part II. The upd-opd transition onto a go...